WebThis factor, called TTAGGG repeat factor (TRF), is present in nuclear extracts of human, mouse, and monkey cells. TRF from HeLa cells was characterized in detail by … WebJul 13, 2024 · The human telomere terminal transferase enzyme is a ribonucleoprotein that synthesizes TTAGGG repeats. Cell 59, 521–529 (1989). Article CAS PubMed Google Scholar ...
Human telomeres contain two distinct Myb–related proteins
To establish global binding profiles of TRF1 and TRF2 (collectively named the TRF proteins), we performed a ChIP-Seq analysis with one antibody specific for TRF1 and two antibodies specific for TRF2 (one monoclonal or TRF2m, one polyclonal or TRF2p). We used the BJ-HELTRasmc tumor cell line because … See more To validate the TRF binding sites identified by ChIP-Seq, we performed independent ChIP experiments with TRF1 and TRF2m antibodies followed by qPCR … See more To determine the type of extratelomeric DNA bound by TRF1 and TRF2, the TRF binding sites were analyzed with a de novo consensus motif prediction software … See more The same motif prediction analysis also identified sequences derived from a consensus consisting of repetitions of the CCATT pentamer 32, which is found in … See more TRF binding sites were preferentially located less than 100 kb from coding sequences (genic regions) (Figure 5A). The 43 genes located proximal to or containing … See more WebOct 1, 1997 · Human telomeres are composed of long arrays of TTAGGG repeats that form a nucleoprotein complex required for the protection and replication of chromosome ends. One component of human telomeres is ... d3a bomber
Replication of circular and linear SV40-based plasmids in monkey cells
WebMar 12, 2024 · Cancer cells have acquired the ability to overcome their fate of senescence via telomere length maintenance mechanisms, ... The human telomere terminal transferase enzyme is a ribonucleoprotein that synthesizes TTAGGG repeats. Cell. 1989;59(3):521–9. WebJul 1, 2015 · In cancer cells, telomere length maintenance occurs largely via the direct synthesis of TTAGGG repeats at chromosome ends by telomerase, or less frequently by the recombination-dependent alternative lengthening of telomeres (ALT) pathway. The latter is characterized by the atypical clustering of tel … WebNov 3, 1989 · I have identified an activity in crude HeLa cell extracts that satisfies the requirements for a human telomere terminal transferase or telomerase. ... The human … d3a headphone amplifier